Genotyping of Salmonella enterica strains from animal and human origin using three molecular techniques

This study aims to characterize different Salmonella enterica subsp molecularly. strains (n=49) were isolated from human gastrointestinal cases in the Tolima region and poultry Santander regions using PCR-RFLP, PCR-ribotyping, PCR-SSCP. The band patterns obtained with each technique analyzed by building dendrograms based on Unweighted Pair Group Method Arithmetic mean (UPGMA) method Dice coefficient. On other hand, discriminatory power of was assessed Simpson's index. genetic profiles gnd gene AciI restriction enzyme PCR-SSCP carried out groEL allowed inter-and intraserovar differentiation. Finally, PCR-ribotyping exhibited highest (0.8571). In conclusion, we show three PCR-based genotyping methods providing an alternative for identifying similarities differences within geographic biological regions.